A Collection of Diagnostic Protocols For Testing Coronavirus

• Pasteur Institute’s Real-Time RT-PCR protocol for detecting SARS-CoV2 using primers against two RNA-dependent-RNA-polymerase targets (IP2 and IP4)

         Protocol: https://www.who.int/docs/default-source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-pasteur-paris.pdf?sfvrsn=3662fcb6_2

• CDC’s protocol for Real-Time RT-PCR in detecting coronaviruses in upper and lower respiratory specimens of infected individuals – Primers designed against viral nucleocapsid gene (N) 

          Protocol: https://www.fda.gov/media/134922/download

• Real-Time RT PCR robust workflow developed by European researchers by targeting RdRp, envelope (E) and nucleocapsid (N) genes of SARS-CoV2

         Protocol: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988269/pdf/eurosurv-25-3-5.pdf

• FDA approved Primerdesign Ltd COVID-19 genesis  Real-Time PCR assay

          Protocol: https://www.fda.gov/media/136309/download

• A faster, specific and cost-effective CRISPR-based diagnostic test strip  – SHERLOCK Assay

        Protocol: https://www.broadinstitute.org/files/publications/special/COVID-19%20detection%20(updated).pdf

Video Tutorial of the Assay:

https://www.youtube.com/watch?v=ZOoUIlLmxf4&feature=emb_title

Assay design resources for 67 viruses:

https://adapt.sabetilab.org/covid-19/

Press release:

https://www.broadinstitute.org/news/enabling-coronavirus-detection-using-crispr-cas13-open-

access-sherlock-research-protocols-and

• A protocol for rapid detection of SARS-CoV2 using CRISPR diagnostics: SARS-CoV2 DETECTR

          Protocol: https://mammoth.bio/wp-content/uploads/2020/03/Mammoth-Biosciences-A-protocol-for-rapid-detection-of-SARS-CoV-2-using-CRISPR-diagnostics-DETECTR.pdf

• Another approved protocol from Ministry of Public Health, Thailand for COVID-18 detection

          Protocol: https://www.who.int/docs/default-source/coronaviruse/conventional-rt-pcr-followed-by-sequencing-for-detection-of-ncov-rirl-nat-inst-health-t.pdf?sfvrsn=42271c6d_4

• National Institute of Infectious Diseases, Japan RT-PCR protocol based on nucleocapsid genes of coronaviruses

          Protocol: https://www.who.int/docs/default-source/coronaviruse/method-niid-20200123-2.pdf?sfvrsn=fbf75320_7

• Hong Kong University’s Real time RT-PCR protocol based on nucleocapsid genes of coronaviruses

         Protocol: https://www.who.int/docs/default-source/coronaviruse/peiris-protocol-16-1-20.pdf?sfvrsn=af1aac73_4

• CDC’s guidelines in handling, collecting and testing of clinical samples 

         Protocol: https://www.cdc.gov/coronavirus/2019-nCoV/lab/guidelines-clinical-specimens.html

• FDA approved protocol for serological testing for faster detection of  COVID-19: Cellex qSARS-CoV2 IgG/IgM Rapid Test

          Protocol: https://www.fda.gov/media/136625/download

          Press release: https://www.fda.gov/news-events/press-announcements/coronavirus-covid-19-update-serological-tests

• A review on updates diagnostic techniques for coronaviruses

        Protocol:: https://www.tandfonline.com/doi/pdf/10.1080/22221751.2020.1745095?needAccess=true

• A 15-minute nanotechnology based coronavirus screening kit 

        Protocol:: https://sonanano.com/sona-develops-rapid-screening-test-for-coronavirus/

• EU and FDA approved Biolidics 10-minute antibody detection kit using whole blood/plasma/serum samples 

         Protocol video: https://www.youtube.com/watch?time_continue=68&v=stqsQgrUoQA&feature=emb_logo

        Protocol:: https://www.biolidics.com/2019-ncov-igg-igm-antibody-detection-kit

• Chest CT scans being viewed as an alternative, reliable diagnostic tool for COVID-19

           Protocol: https://www.statnews.com/2020/04/16/ct-scans-alternative-to-inaccurate-coronavirus-tests/?utm_source=STAT+Newsletters&utm_campaign=fffd3e2a6e-Daily_Recap&utm_medium=email&utm_term=0_8cab1d7961-fffd3e2a6e-152429581

• ‘Probe-free detection’ assay developed by IIT Delhi researchers for affordable COVID-19 detection in India

        Protocol: https://www.deccanherald.com/science-and-environment/iit-delhi-researchers-develop-affordable-test-for-covid-19-816358.html

• Links to different diagnostic test kits being currently developed across the globe:

Courtesy:  Sridhar Sivasubbu, Sheetal Gandotra, and Vivek Rao Labs at CSIR-IGIB

     Protocol: Check This Google Document

Last Updated April 17, 2020

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Priyanka Lahiri

Priyanka is hailing from West Bengal, India.  She completed her undergraduate degree in Microbiology from St. Xaviers College, Kolkata and her masters degree in Biochemistry from University of Calcutta. For her Ph.D, she moved to Indian Institute of Science (IISc), where she researched in engineering novel beta-hairpin peptides and evaluated their applicability in generating potent, non-cytotoxic and bioavailable antimicrobial peptides. Currently, she is a Research Associate at IISc. Outside lab, she is a research buff, who is always inquisitive to learn about new and innovative research. Her work in CoronaCures as a volunteer researcher, is her way to contribute in spreading awareness about COVID-19 about latest treatment and vaccines development.

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